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. 2020 Aug;8(15):933. doi: 10.21037/atm-20-3871

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2020 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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The combination of GSK3 and an HDAC inhibitor also increases the activity of common promoter as well as a NSE promoter. (A,B) Representative IVIS images and quantification of luciferase expression in induced neural stem cells (hiNSCs) transfected with SV or NSE plasmids. This experiment was performed in two different types of iNSCs; hiNSCs were maintained under hypoxia for 24 h after CHIR99021- and valproic acid-treatment. This result indicates that the combination of two small molecules is suitable for a common promoter and is not specific to the NSE promoter. *, P<0.05. C, CHIR99021; V, valproic acid.