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. 2020 Aug;8(16):1007. doi: 10.21037/atm-20-5332

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2020 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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Inhibited SNHG22 could suppress the progression of ESCC cells. (A) Expression of SNHG22 was assessed in the human esophageal epithelial cell (HET-1A) and esophageal squamous cell carcinoma cells (Kyse-150, TE-10, and Eca109) via the qRT-PCR assay. (B) The inhibition efficiency of SNHG22 was verified by the qRT-PCR assay. (C,D) TE-10 and Eca109 cell proliferation were detected by CCK8 and colony formation assay. (E,F) Cell cycle progress and apoptosis of TE-10 and Eca109 cells are probed by flow cytometry analysis. (G,H) Cell autophagy of TE-10 and Eca109 cells was analyzed by immunofluorescence assay and western blot assay. **, P<0.01. SNHG22, small nucleolar RNA host gene 22; ESCC, esophageal squamous cell carcinoma; qRT-PCR, quantitative real-time PCR.