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. 2020 Aug;8(16):1007. doi: 10.21037/atm-20-5332

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2020 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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SNHG22/miR-429 /SESN3 axis was proved in ESCC cells. (A) Venn diagram was drawn to show potential mRNAs. (B) qRT-PCR assay was implemented to search which mRNA was highly expressed in TE-10 and Eca109 cells and could be affected by SNHG22 and miR-429. (C) SESN3 expression was evaluated in the human esophageal epithelial cell (HET-1A) and esophageal squamous cell carcinoma cells (Kyse-150, TE-10, and Eca109) via the qRT-PCR assay. (D) The binding site of SESN3 and miR-429 was presented according to ENCORI. (E) Luciferase reporter assay was conducted to verify the binding relationship in SESN3 and miR-429. (F) RIP assay was used to prove the binding of SNHG22, miR-429, and SESN3. **, P<0.01. SNHG22, small nucleolar RNA host gene 22; ESCC, esophageal squamous cell carcinoma; qRT-PCR, quantitative real-time PCR.