Figure 2.

Effect of TLE2 on the proliferation, invasion, and cell cycle of pancreatic cancer cells in vitro. (A,B) The expression of TLE2 in pancreatic cell lines was assessed by qRT-PCR (A) and western blotting (B), with GAPDH used as an internal control. (C) TLE2 protein expression was validated in pancreatic cancer cell lines with the indicated treatments by western blotting. (D,E) Cell growth in PANC-1 cells (D) and MiaPaCa-2 cells (E) was assessed by MTT assays at an absorption value of 570 nm (OD570). (F,G) Flow cytometry was used to detect cell cycle distribution in the PANC-1 cells (F) and MiaPaCa-2 cells (G). The proportions of cells in each phase are displayed in the histograms. (H) Cell invasion was evaluated using Transwell matrigel invasion assays. As showed in the histograms (I,J), the numbers of cells per HPF that passed through the Transwell chambers were counted, normalized, and compared. TLE, transducin-like enhancer of split; HPF, high power field.