Fig. 5.

Analysis of the frequency of stop codon substitution in viral proteins and viral load. a Comparison of stop codon frequency in the L protein at position 986. P values were calculated with the one-sided Wilcoxon rank sum test, with the average stop codon frequency at position 986 in the EBOV L protein and compassion between hospitalised fatal and hospitalised survived cases. b A Q-Q plot was used to compare the distribution of the stop codon at position 986 in the L protein between hospitalised fatal and hospitalised survived cases. The values below the line suggest the data, i.e. the presence of the stop codon, was more frequent in the hospitalised survivor cases. c The summary of stop codon frequency in all EBOV proteins compared between hospitalised fatal and hospitalised survived cases. d A two-sided Spearman rank correlation test was used to estimate the correlation of stop codon frequency with viral load (1/Ct) at position 986, where the R value is the correlation coefficient ranging from − 1 (strong negative correlation) to + 1 (strong positive correlation), and P is the P value for this test. In b, d and e, only the samples with at least one stop codon are shown. In a, c and d, only the samples with at least one stop codon are shown