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Lysates of PPP and the buffy coat layer but not heated PPP provoked nuclear translocation of Smad2/3. Gingival fibroblasts were exposed for 1 h to 30% of the lysates prepared from PPP, heated PPP (75 °C for 10 min, Alb-gel), and buffy coat (BC). For red clot, 5% of the lysates was used. Immunofluorescence revealed the nuclear translocation of Smad2/3 by lysates prepared from PPP and the buffy coat (BC) but not when cells were exposed to lysates of heated PPP and red clot.
