Figure 2.

Characteristics of ATF₂₄ modified PEGylated liposomes. (A) Schematic illustration of ATF₂₄-PEG-Lipo-β-E. (B) LC-ESI-MS spectra of the ATF₂₄ peptides. (C) MALDI-TOF-MS spectrum of DSPE-PEG₂₀₀₀-ATF₂₄. (D) Morphology of ATF₂₄-PEG-Lipo-β-E by TEM (2,000×). (E) Particle size of ATF₂₄-PEG-Lipo-β-E. (F) Zeta potential of ATF₂₄-PEG-Lipo-β-E. (G) FTIR spectrum of (a) β-E, (b) the physical mixture of (β-E/soybean lecithin/cholesterol/DSPE-PEG₂₀₀₀), (c) PEG-Lipo, (d) PEG-Lipo-β-E and (e) ATF₂₄-PEG-Lipo-β-E. (H) Differential scanning chromatogram of ATF₂₄-PEG-Lipo-β-E. (I) In vitro release behaviors of free β-E, PEG-Lipo-β-E and ATF₂₄-PEG-Lipo-β-E in 75% ethanol at 37 °C. ***P < 0.001.