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. 2020 Aug 15;17(3):707–725. doi: 10.20892/j.issn.2095-3941.2020.0056

Figure 3.

Figure 3

SHP2 knockout delays G1-to-S phase transition through downregulation of Cyclin D1 abundance in breast cancer cells. (A) SHP2 knockout increased the percentage of G1 phase cells and decreased the percentage of G2/S phase cells. The cell cycle was evaluated through flow cytometry assays. The percentages of cells in each cell cycle phase are shown as mean ± SD from 3 independent experiments (****P < 0.0001). (B) EdU incorporation assays showed that the proportion of cells in the S phase was lower in SHP2 knockout cells than control cells. Data are presented as mean ± SD (**P < 0.01, ****P < 0.0001). (C) SHP2 knockout markedly decreased the protein level of Cyclin D1, whereas the expression of Cyclin B1 and Cyclin E1 was not altered. (D) SHP2 knockout decreased the mRNA expression of Cyclin D1. Quantitative PCR analysis of the mRNA expression of Cyclin B1, Cyclin D1, and Cyclin E1 in control and SHP2 knockout cells. Data are presented as mean ± SD. Statistical analysis was carried out with one-way ANOVA (*P < 0.1, **P < 0.01).