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. 2020 Aug 15;12(8):4264–4276.

Figure 4.

Figure 4

SPR064 induced apoptosis in EC cells. The ECC-1 and Hec-1a cell lines were treated with varying concentrations of SPR064 and PTX for 16 hours. Apoptosis was detected using the Annexin-V FITC assay and cleaved caspase 3 ELISA assay in both cell lines. SPR064 and PTX increased Annexin V expression and cleaved caspase 3 activity in a dose dependent manner in the ECC-1 and Hec-1a cells (A and B). Western blotting demonstrated that treatment with SPR064 for 24 hours decreased the expression of MCL-1 and BCL-XL in both cell lines (C).