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. 2020 Aug 15;12(8):4456–4466.

Figure 3.

Figure 3

SNHG9 upregulated CAPRIN1 through direct interaction. A. StarBase v2.0 (http://starbase.sysu.edu.cn/) scanning found potential target proteins of SNHG9. B-D. Biotinylated RNA pulldown assay showed SNHG9-DNA-antisense biotin probes enriched more SNHG9 and CAPRIN1 protein than SNHG9-DNA-sense probes in A549/DDP and H1299/DDP cell lysates. E-G. RNA immunoprecipitation assay showed anti-CAPRIN1 antibody captured more CAPRIN1 protein and SNHG9 compared with control IgG in A549/DDP and H1299/DDP cell lysates. H. Western blot showed sh-SNGH9#1/sh-SNGH9#2 decreased the protein level of CAPRIN1 in A549/DDP and H1299/DDP cells. I. Western blot showed forced expression of SNGH9 (SNGH9 overexpressing plasmid (SNGH9-oe) transfection) increased the protein level of CAPRIN1 in A549/DDP and H1299/DDP cells. Actin was detected as control for western blot. *P<0.05; **P<0.01.