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. 2020 Aug 15;12(8):4456–4466.

Figure 4.

Figure 4

CAPRIN1 inhibition enhanced DDP sensitivity in DDP-resistant NSCLC. In A549/DDP and H1299/DDP cells, (A) RT-qPCR showed si-CAPRIN1#1, si-CAPRIN1#2 transfection decreased CAPRIN1 mRNA level; (B) Western blot showed si-CAPRIN1#1, si-CAPRIN1#2 transfection decreased CAPRIN1 protein level; (C and D) MTT assay showed CAPRIN1 knockdown decreased cell viabilities during a period of 4 days with the treatment of DDP (5 μM); (E and F) Cell colony formation assay showed CAPRIN1 knockdown decreased cell colony formation with the treatment of DDP (5 μM); (G) CAPRIN1 knockdown decreased IC50 for DDP; (H) Caspase-3 activity assay and (I) DNA fragmentation assay showed SNGH9 knockdown increased cell apoptosis after the treatment of DDP (5 μM). *P<0.05; **P<0.01.