Figure 5.

CAPRIN1 rescued the deceased DDP-resistance due to SNHG9 knockdown in DDP-resistant NSCLC. A549/DDP cells was co-transfected with sh-SNHG9#1/sh-Ctrl and CAPRIN1-oe/Vector. (A) Protein level of CAPRIN1 was examined by western blot. Actin was detected as control. (B) MTT assay, (C) Cell colony formation assay, (D) IC₅₀ analyzation, (E) Caspase-3 activity assay and (F) DNA fragmentation assay showed SNGH9 knockdown decreased cell viabilities, cell colony formation, IC₅₀ for DDP, increased cell apoptosis respectively, and forced expression of CAPRIN1 abolished these changes. *P<0.05; **P<0.01.