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Primers for VEGF, IL-8, HDAC2 and GAPDH
| Primer | Sequences | Length of primer (bp) | Length of product (bp) | Annealing temperature (°C) |
|---|---|---|---|---|
| VEGF F | CGAGTACATATTCAAGCCTTCC | 22 | 207 | 57.1 |
| VEGF R | CTTGCTCTGTCTTTCTTTGGTC | 22 | ||
| IL-8 F | CTGTTGGTCAGGCCATGAGT | 20 | 125 | 60.1 |
| IL-8 R | AAAGTGCTTCCATGTGCCCT | 20 | ||
| HDAC2 F | AGGAGACTTGAGGGATATTGG | 21 | 228 | 56 |
| HDAC2 R | CATTTAGCATGACCTTTGACTG | 22 | ||
| GAPDH F | GCCGCCCAGAACATCAT | 17 | 192 | 58.2 |
| GAPDH R | TGCCTGCTTCACCACCTT | 18 |
