Figure 2.

Relative expression of CXCR5 mRNA and protein levels in peripheral blood mononuclear cells (PBMCs) of patients with chronic spontaneous urticaria (CSU) and healthy controls (HC). A. The CXCR5 mRNA expression levels were determined by quantitative real-time (qRT)-PCR, with β-actin as an internal reference gene. B and C. The CXCR5 protein expression levels were determined by western blot, with GAPDH as the endogenous control. B. Representative data (n = 4) by the western blot analysis of total protein samples from PBMCs of CSU patients and HC. C. The CXCR5/GAPDH in PBMCs were calculated from the western blot results. Vertical bars represent SEM. Significance between two groups was evaluated with a two-tailed Student’s t-test and indicated by p value (*P < 0.05, ***P < 0.001).