Figure 3.

Effects of FOXM1 on PDAC cell proliferation and migration were mediated by regulating ATX expression. A. 10 μL of ATX-specific siRNAs (siATX-1 and siATX-2) were transfected into PANC-1 cells, respectively. And after incubation for 48 h, the efficiencies of siATX-1 and siATX-2 were verified by western blots. B. PANC-1 and SW1990 cells were transfected with pFOXM1 or siATX-1 as indicated, and after 24 h, CCK-8 assays were performed using the indicated PANC-1 and SW1990 cells and cell viability was measured at the indicated time points. C. PANC-1 and SW1990 cells with stable expression of FOXM1 were transfected with siATX-1/siNC as indicated, and colony formation assays were performed 24 h after transfection. D. PANC-1 and SW1990 cells with stable expression of FOXM1 were transfected with siATX-1/siNC as indicated, and cell migration assays were performed 24 h after transfection. *P < 0.05, **P < 0.01.