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. 2020 Aug 15;12(8):4478–4487.

Figure 4.

Figure 4

ATX regulated FOXM1 expression via inhibiting the Hippo/YAP signaling. (A and B) 48 h after transfection with siATX-1, proteins or RNAs were isolated from PANC-1 cells and western blots (A) and qPCR (B) analyses were performed to investigate the effects of ATX knockdown on FXOM1 protein and mRNA expression. (C and D) 42 h after transfection with siATX-1, PANC-1 cells were treated with a Hippo signaling-specific inhibitor XMU-MP-1 (3 μM) for 6 h, and western blots (C) and qPCR analyses (D) were performed to examine the expression changes of FOXM1 at protein and mRNA levels. (E) PANC-1 cells were co-transfected with siATX-1/siNC and pFOXM1-2496 for 18 h, and then treated with XMU-MP-1 (3 μM) for 6 h. Finally dual luciferase reporter assays were performed to investigate the changes of FOXM1 promoter activity. *P < 0.05, **P < 0.01.