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. 2020 Aug 15;12(8):4354–4370.

Figure 5.

Figure 5

(A) Examination of DEGs in RNA-seq by qRT-PCR. The upregulated genes included PLK5, SLIT2, CDKN1C, CDKN2A, NGFR, netrin 1, neurocan, neurofilament heavy, neurofilament medium, neurofilament light, neurogenin 2, neuronal Per-Arnt-Sim (PAS) domain protein 1, and SOX11, while CDK6 and CDK1 were downregulated (n = 3). (B) Western blot detected that the levels of ASCL1, CDK4, and CDK6 were decreased while those of SOX11, PLK5, and SLIT2 were increased after Fasudil stimulation (n = 3). (C) Representative gross image showed Fasudil inhibited xenograft SCLC tumor growth (n = 5). (D-G) HE staining showed tumors in the Fasudil group had more abundant interstitial substances and loose cell arrangement. Gordon-Sweet staining showed more reticular fibers in the Fasudil group (D). TUNEL assay showed that Fasudil could promote tumor apoptosis (D, E), with a significant reduction in tumor volume (F) and weight (G) (magnification × 200). Data were presented as mean ± SEM; *P < 0.05, **P < 0.01, and ***P < 0.001.