Figure 3.

Further identification of hub genes using LASSO regression analysis. A, B. To further select significant prognostic metastasis-related biomarkers, LASSO Cox regression was performed to further search for hub genes associated with metastasis and to narrow the scope of the panel, suggested that a total of 31 genes, including C1S, PLOD2, ADAMTS9, AK1, CHD2, DCAF8, DNAJC10, DPP3, DPYSL3, GSTM3, H2AFX, HBA1, RUNX1, MAMDC4, MFAP3L, MINA, OSBPL1A, PIAS2, RBBP6, RCOR3, RHOBTB3, SLC19A1, SORBS2, SPTAN1, SPTBN1, SYT13, TPM4, TPX2, TRIB3, UGGT1 are significant weighted prognostic factors. C. GO function annotations of 31 selected genes. Functional annotation of these genes revealed they were primarily enriched in the terms as follows: regulation of actin filament capping, non-membrane-bounded organelle, sarcomere, myofibril, organelle lumen, nucleolus, actin cytoskeleton, and nuclear lumen.