Figure 3.

Elabela alleviated apoptosis triggered by cardiac ischemia/reperfusion. A. TUNEL-positive (green) cardiomyocytes were evaluated by double staining with TUNEL (green) and 4’, 6’-diamidino-2-phenylindole (blue). Scale bar: 20 μm. B. Expression levels of Bcl-2, Bax, cleaved-caspase3, caspase-3 and Cyt-c were measured using western blotting. IRI, ischemia reperfusion injury. All results were obtained from at least three independent experiments. All numerical data were presented as the mean ± standard deviation. *P < 0.05, **P < 0.01, ***P < 0.001 versus sham group; #P < 0.05 versus IRI group.