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. Author manuscript; available in PMC: 2020 Sep 7.
Published in final edited form as: Immunohorizons. 2020 Feb 18;4(2):82–92. doi: 10.4049/immunohorizons.1900089

FIGURE 3. Splenectomy alters the expression of CCR4 and CCR6 on T cells and myeloid cells.

FIGURE 3.

Mass cytometric analysis of lysed whole blood from patients before and after splenectomy. (C) The timing of the postsplenectomy blood draws from each patient. Files were normalized and gated on single CD45+ cells prior to analysis. (A) PhenoGraph analysis of CD45+ events comparing pre- and postsplenectomy samples from all five patients, colored by cellular phenotype (cell populations were identified based on the markers in Supplemental Table III). (B) The frequencies of PhenoGraph-defined clusters comparing the values of each cell type between pre- and postsplenectomy samples from each patient. (C) Comparison of the frequencies of PhenoGraph-defined clusters between pre- and postsplenectomy samples from each patient, including the number of days postsplenectomy the blood draw occurred. (D) The CD45+ events from pre- and postsplenectomy colored based on the expression of either CCR6 (top) or CCR4 (bottom). (E) Quantification of the mean metal intensity (relative expression level) of CCR6 (top) or CCR4 (bottom) compared between the designated cellular phenotypes and pre- and postsplenectomy samples compared across the designated cellular phenotypes.