Table 1.
The information and parameters for the expression of 27 M. hyopneumoniae fusion proteins in this study.
| Gene | Gene size | Mutant site | Restriction sites | MWa of pure protein (kDa) | MW of GST fusion protein (kDa) | Vector | Recipient strain | Induction temperature (°C) |
|---|---|---|---|---|---|---|---|---|
| mhp104 | 984 bp | 199–201, 388–360, 628–630 | BamH Ⅰ, Xho Ⅰ | 37 | 63 | pGEX-6P-1 | BL21 (DE3) | 30 |
| mhp153 | 1470 bp | 181–183, 481–483, 574–576, 1105–1107, 1405–1407, 1435–1437, 1459-1461 | BamH Ⅰ, Xho Ⅰ | 54 | 80 | pGEX-6P-1 | BL21 (DE3) | 16 |
| mhp156 | 1062 bp | 133–135, 169–171, 193–195, 235–237, 256–258 | BamH Ⅰ, Sal Ⅰ | 41 | 67 | pGEX-6P-1 | XL-1 Blue | 16 |
| mhp228 | 561 bp | 394–396 | BamH Ⅰ, Xho Ⅰ | 21 | 47 | pGEX-6P-1 | BL21 (DE3) | 16 |
| mhp252 | 1071 bp | 268–270 | BamH Ⅰ, Xho Ⅰ | 39 | 65 | pGEX-6P-1 | BL21 (DE3) | 30 |
| mhp265 | 1125 bp | 304–306, 379–381, 586–588, 592–594, 625–627, 811–813, 889–891, 964–966 | BamH Ⅰ, Xho Ⅰ | 42 | 68 | pGEX-6P-1 | BL21 (DE3) | 16 |
| mhp299 | 1383 bp | 139–141, 1096–1098, 1135–1137 | BamH Ⅰ, Sal Ⅰ | 51 | 77 | pGEX-6P-1 | BL21 (DE3) | 30 |
| mhp322 | 1173 bp | 790–792 | BamH Ⅰ, Xho Ⅰ | 44 | 70 | pGEX-6P-1 | BL21 (DE3) | 30 |
| mhp336 | 1473 bp | 82–84, 490–492, 925–927, 1159–1161, 1216–1218 | BamH Ⅰ, Sal I | 58 | 84 | pGEX-6P-1 | BL21 (DE3) | 16 |
| mhp351 | 1461 bp | 268–270, 1114–1116 | BamH Ⅰ, Xho Ⅰ | 58 | 84 | pGEX-6P-1 | BL21 (DE3) | 16 |
| mhp364 | 1599 bp | 676–678, 979–981, 1456–1458 | BamH Ⅰ, Xho Ⅰ | 61 | 87 | pGEX-6P-1 | BL21 (DE3) | 30 |
| mhp367 | 1575 bp | 571–573, 736–738, 856–858, 868–870, 904–906, 1159–1161, 1207–1209 | BamH Ⅰ, Xho Ⅰ | 61 | 87 | pGEX-6P-1 | BL21 (DE3) | 30 |
| mhp379 | 933 bp | 223–225, 310–312 | BamH Ⅰ, Xho Ⅰ | 36 | 62 | pGEX-6P-1 | BL21 (DE3) | 16 |
| mhp390 | 1815 bp | 1066–1068, 1351–1353, 1576–1578 | BamH Ⅰ, Xho Ⅰ | 68 | 94 | pGEX-6P-1 | BL21 (DE3) | 30 |
| mhp424 | 1149 bp | 10–12, 997–999 | BamH Ⅰ, Xho Ⅰ | 44 | 70 | pGEX-6P-1 | BL21 (DE3) | 30 |
| mhp462 | 1380 bp | 895–897, 1193–1095, 1138–1140 | BamH Ⅰ, Xho Ⅰ | 51 | 77 | pGEX-6P-1 | BL21 (DE3) | 16 |
| mhp465 | 942 bp | 319–321, 421–423, 745–747 | BamH Ⅰ, Xho Ⅰ | 37 | 63 | pGEX-6P-1 | BL21 (DE3) | 16 |
| mhp472 | 729 bp | 19–21, 622–624 | BamH Ⅰ, Xho Ⅰ | 27 | 53 | pGEX-6P-1 | BL21 (DE3) | 16 |
| mhp477 | 1539 bp | 1258–1260 | BamH Ⅰ, Xho Ⅰ | 58 | 84 | pGEX-6P-1 | BL21 (DE3) | 16 |
| mhp483 | 804 bp | 319–321 | BamH Ⅰ, Xho Ⅰ | 31 | 57 | pGEX-6P-1 | BL21 (DE3) | 16 |
| mhp488 | 1215 bp | 430–432, 970–972 | BamH Ⅰ, Xho Ⅰ | 44 | 70 | pGEX-6P-2 | XL-1 Blue | 30 |
| mhp504 | 1848 bp | 580–582, 709–711, 1801–1803 | BamH Ⅰ, Xho Ⅰ | 66 | 92 | pGEX-6P-1 | BL21 (DE3) | 30 |
| mhp511 | 1260 bp | 208–210, 301–303, 760-762 | BamH Ⅰ, Xho Ⅰ | 46 | 72 | pGEX-6P-1 | BL21 (DE3) | 30 |
| mhp535 | 1677 bp | 388–390, 565–567, 928–930, 943–945, 1258–1260, 1270–1272, 1480–1482, 1489-1491 | BamH Ⅰ, Xho Ⅰ | 66 | 92 | pGEX-6P-1 | BL21 (DE3) | 30 |
| mhp623 | 1356 bp | 19–21, 295–297, 412–414, 454–456, 520–522, 592–594, 736-738 | BamH Ⅰ, Xho Ⅰ | 50 | 76 | pGEX-6P-1 | BL21 (DE3) | 30 |
| mhp677 | 1884 bp | 322–324, 553–555, 925-927 | BamH Ⅰ, Xho Ⅰ | 71 | 97 | pGEX-6P-1 | BL21 (DE3) | 30 |
| mhp682 | 1566 bp | 1045–1047, 1315–1317, 1348–1350, 1444-1446 | BamH Ⅰ, Xho Ⅰ | 60 | 86 | pGEX-6P-1 | BL21 (DE3) | 30 |
a MW, molecular weight.