Table 3.

The predictive results of computational tools, comprehensive predictive localization and the manually curated localization.

Protein name	Phobiusa	TMHMM Server v. 2.0	LipoP 1.0b	TatP 1.0c	SignalP 5.0d	Comprehensive predictive localization	Reported localization	Manual curation
Mhp104	Me	M		1–22		M		M
Mhp153	Ef	E				E	M [25, 26]	M (Identified)
Mhp156	M	E				M	M [25]	M (Identified)
Mhp228	E	E				E	E [27]	E (Identified)
Mhp252	Cytoplasm	E				Cytoplasm	M [33]	M (Identified)
Mhp265	E	E				E	M [25]	M (Identified)
Mhp299	E	E				E	M [25, 26]	M (Identified)
Mhp322	E	E				E	M [25]	M (Identified)
Mhp336	1–25 (E)	E (surface)	1–24		1–24	E		E
Mhp351	1–33 (E)	M (surface)	1–33			M (surface)		M
Mhp364	1–30 (E)	E	1–29		1–29	E	M [25], E [27]	M/E (Identified)
Mhp367	1–22 (E)	E	1–21		1–21	E	M [25]	M (Identified)
Mhp379	1–27 (E)	E	1–24		1–24	E	M [25]	M (Identified)
Mhp390	1–25 (E)	E	1–23		1–23	E	M [25]	M (Identified)
Mhp424	1–28 (E)	E	1–27		1–27	E		E
Mhp462	E	E				E	M [34]	M (Identified)
Mhp465	1–25 (E)	M (surface)	1–23		1–23	M (surface)	M [25]	M (Identified)
Mhp472	E	E				E	M [25]	M (Identified)
Mhp477	E	E				E	M [25]	M (Identified)
Mhp483	M (multiple transmembrane)	M (multiple transmembrane)	1–39			M (multiple transmembrane)		M (multiple transmembrane)
Mhp488	E	E				E	M [25]	M (Identified)
Mhp504	E	E				E	M [25, 26]	M (Identified)
Mhp511	1–29 (E)	M (surface)	1–30		1–30	M (surface)	M [25,26]	M (Identified)
Mhp535	1–35 (E)	E	1–34		1–34	E		E
Mhp623	1–27 (E)	E	1–23		1–28	E	M [25, 26], E [27]	M/E (Identified)
Mhp677	1–27 (E)	E	1–29		1–29	E	M [25, 26], E [27]	M/E (Identified)
Mhp682	1–29 (E)	M (surface)	1–25		1–25	M (surface)	M [25], E [27]	M/E (Identified)

a-d Position of the signal peptide in the protein.

e M: membrane.

f E: extracellular.