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. 2020 Jul 24;75(9):754–763. doi: 10.1136/thoraxjnl-2019-214000

Figure 5.

Figure 5

Mast cell (MC) survival in co-culture with fibroblasts is mediated largely through stem cell factor (SCF) and is inhibited by nintedanib. MCs were cultured alone or with normal human lung fibroblasts (NHLFs) in media lacking SCF or IL-6 growth factors for 21 days. (A) At each time point, cells were stained with a viability dye and anti-ckit APC mIgG1 and ckit-positive MCs quantified by flow cytometry. There is a survival benefit to MCs co-cultured with NHLFs, and this is inhibited in the presence of 67 nM anti-SCF. Inclusion of 30nM nintedanib in the co-culture has a similar effect. Data plotted as mean and SD of 3 separate experiments (2 MC donors). (B) Experimental time course plotted. (C) Day 14 data plotted as bar chart with mean and SD and showing percentage inhibition relative to isotype/vehicle control. Groups were compared by ANOVA with Sidak’s post hoc test for multiple comparisons. CBMC, cord blood–derived mast cell; FSC-SSC, forward scatter versus side scatter; LD, live dead viabilty dye.