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. 2020 Aug 20;23(9):101472. doi: 10.1016/j.isci.2020.101472

Figure 8.

Figure 8

Reduced Amount of phosphorylated FAK (PFAK) and Altered Cytoskeleton in Adamts18−/− Lungs

(A) IHC results of Tyr397-PFAK and total FAK of E14.5 lungs. Scale bar, 50 μm. Determination of the intensity of FAK staining and the percentage of Tyr397-PFAK-positive epithelial cell was performed with Image Pro Plus. Scale bar, 100 μm.

(B) GTP-bound RhoA in E15.5 lungs was precipitated with Rhotekin and analyzed by western blotting for RhoA. RhoA-GTP was quantified relative to total RhoA protein.

(C) Representative images of anti-TMSB10 IHC at E14.5. Quantification of TMSB10-positive areas was performed with Image Pro Plus. Scale bar, 100 μm.

(D) Representative images of phalloidin-stained E14.5 lung sections. White dotted lines indicate the boundary of distal epithelium. B, bronchiole. Scale bar, 20 μm.

(E) Transwell analysis of mouse embryonic fibroblasts (MEFs) from Adamts18+/+ or Adamts18−/− mice. Data are expressed as mean ± SEM (n = 3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

See also Table S2.