Fig. 4.

GR gene delivery attenuated Klotho deficiency induced renal fibrosis. (A) Representative photomicrographs of Masson's trichrome-stained kidney sections. Arrows indicate trichrome-positive collagenous components in cortical interstitium (blue staining). Semi-quantification of the area fraction of collagen deposition in kidneys. Scale bars, 50 μm. (B) Representative Western blot bands and quantitative analysis of type I collagen. The relative protein expression was normalized to β-actin first and then calculated as fold changes of the controls (WT + PBS). n = 4–6 mice/group. *P < 0.05 **P < 0.01 vs WT + PBS; ⁺P < 0.05 ⁺⁺ P < 0.01 vs KL^+/- + AAV-GFP. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)