Fig. 6.

GR gene delivery restored GR protein expression and GSH/GSSG ratio in kidneys of Klotho-deficient mice. (A) Representative photomicrographs of GR immunostaining in kidney sections (brown color). Semi-quantification of GR staining in mice kidneys. Upper and lower photomicrographs represent cortex and medulla area, respectively. Scale bars, 50 μm. (B) Representative Western blot bands and quantitative analysis of GR. The relative protein expression was normalized to β-actin first and then calculated as fold changes of the controls (WT + PBS). (C) GSH/GSSG ratio in kidneys. n = 4–6 mice/group. *P < 0.05 **P < 0.01 vs WT + PBS; ⁺P < 0.05 ⁺⁺ P < 0.01 vs KL^+/- + AAV-GFP. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)