Fig. 10.

Quantification of mRNA decay rates in four dual UTR constructs in E. coli. Escherichia coli clones were first grown for 5 h under induced state with 2 mM m-toluic acid, and afterwards the growth medium was replaced with a fresh medium not containing the inducer, performed for three biological replicates. In total, five samples were taken with 2 min intervals ranging from 0 to 10 min with three technical replicates in each time point. qPCR analyses was performed on the samples and the mRNA decay rates were calculated for the four different dual UTR constructs.