Fig. 9.
Quantification of mCherry expression levels under the control of the AraC/PBAD promoter system in E. coli clones harboring plasmids with TrTn and TnTr dual UTR constructs. Relative mCherry fluorescent intensities were quantified after 5 h and 18 h of overnight (O/N) growth at the induced state with L-arabinose to a final concentration of 0.2%. Fluorescence intensities were determined directly from the cultures and normalized with OD600 values. The fluorescent intensity value for the wtwt dual UTR construct at the uninduced state was arbitrarily set to 1.0. Error bars indicate the standard deviations calculated from three biological and three technical replicates.