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. Author manuscript; available in PMC: 2020 Sep 7.
Published in final edited form as: J Am Soc Mass Spectrom. 2019 May 20;30(9):1663–1678. doi: 10.1007/s13361-019-02225-3

Scheme 1.

Scheme 1.

An example of a workflow for glycan occupancy calculations for N182 glycosite (RLDVVQIN177ENQGN182R, CT26–27 component of gp120 subunit, the missed-cleaved product of the combined [trypsin + chymotrypsin] digest). XIC of the CT26027 [2+] charge state displays several peptides containing N177, N182, and their deamidated analogs. The workflow accounts for the endogenous deamidation and residual glycosylation. The calculations are illustrated using the Table 2 values. The brackets denote the MS peak intensity (counts)