FIG. 3.

Drug toxicities of dornase alfa on peripheral blood mononuclear cells (PBMCs). Three healthy adult PBMC samples were inoculated with dornase alfa in a dose-dependent manner for 48 h before flow cytometric analysis. (a) Flow cytometry plots that show immune cell subtypes (CD19⁺ B, CD3⁺ CD4⁺ T helper, CD3⁺ CD8⁺ cytotoxic T, and CD3⁺ CD56⁺ natural killer T (NKT) cells) and their activation (with alfaCD25 and alfaCD107a). (b) The bar graph that shows the change in average frequency of total CD3⁺ T lymphocytes in the PBMCs after treatment. (c) The bar graphs that show average proportions of the immune cell subtypes and their activation. (d) The flow cytometry plots showing viability of the cells analysed with 7aminoactinomycin D staining in flow cytometry. The bar graph showing viable proportion of the PBMCs treated with dornase alfa. ∗p < 0.05; NS, not significant.