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. 2020 Aug 27;16:2087–2099. doi: 10.3762/bjoc.16.176

Figure 2.

Figure 2

Problems when integrating poorly resolved peaks using FA1/FA2G2S1/A2 and M5 peaks as an example. (A) FA1/FA2G2S1/A2 and M5 had similar peak areas. (B) FA1/FA2G2S1/A2 had a greater peak area than M5. (C) FA1/FA2G2S1/A2 had less peak area than M5. (D) Average peak area and standard deviation (error bars) for 9 electropherograms in A. (E) Average peak area and standard deviation (error bars) for 326 electropherograms in B. (F) Average peak area and standard deviation (error bars) for 56 electropherograms in C. (G). Correlations between 32 Karat and HappyTools/Riemann sums for FA1/FA2G2S1/A2 only (red box peak not picked by 32 Karat), (H) M5 peaks only (red box peak not picked by 32 Karat, and (I) when FA1/FA2G2S1/A2 and M5 peak areas were combined there was excellent correlation between both approaches suggesting 32 Karat integrates the two peaks as a whole.