Figure 4.
Desmin was not present in the myofibers containing smyhc1:tdTomato in the zebrafish embryo EOMs. Ventral and dorsal view showing desmin (magenta) in fast/slow double transgenic (smyhc1:tdTomato (red)/mylz2:GFP (green) positive) embryos at 3 dpf (A–F) and 5 dpf (G–L). Squares indicate approximate area of magnification in panels to the right. Red arrowheads indicate slow smyhc1:tdTom-positive myofibers, green arrowheads indicate fast mylz2:GFP-positive myofibers, and white arrowheads indicate mylz2:GFP/desmin double-positive myofibers. Desmin was only present in mylz2:GFP-positive myofibers and was lacking in smyhc1:tdTomato-positive myofibers. am, anterior mandibulae muscle. Scale bars: 100 µm.