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. 2020 Sep 1;9(10):1. doi: 10.1167/tvst.9.10.1

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Figure 5. — NMJs in the global layer of the zebrafish MR muscle. Longitudinal view of large-diameter fast myofibers in the global layer identified with mylz2:GFP (A, F), treated with α-bungarotoxin (B, G), and merged image of mylz2:GFP/α-bungarotoxin (C, H), desmin immunolabeling (D, I), and merged image of desmin/α-bungarotoxin (E, J). These myofibers displayed single en plaque (A–E) and multiterminal en plaque NMJs (F–J, arrowheads). Arrowheads indicate slightly increased desmin immunolabeling intensity in the vicinity of single (D, E) and multiterminal NMJs (I, J). NMJs on the same myofiber are marked with the same arrowhead type in H–J. n, nucleus. Scale bar: 25 µm.