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. 2020 Sep 1;9(10):1. doi: 10.1167/tvst.9.10.1

Figure 6.

Figure 6.

NMJs in the orbital layer of the zebrafish MR muscle. Longitudinal view of the thinner fast myofibers of the orbital layer labeled with mylz2:GFP (A1, B1), smyhc1:tdTom (C1, D1, E1, F1), α-bungarotoxin (A2, B2, C2, D2, E2, F2), and desmin (A4, B4, C4, D4, E4, F4). Merged image of mylz2:GFP/α-bungarotoxin (A3, B3), smyhc1:tdTom/α-bungarotoxin (C3, D3, E3, F3), and desmin/α-bungarotoxin (A5, B5, C5, D5, E5, F5). Fast myofibers displayed single en plaque motor endings, either lacking (A4, open arrowhead) or containing (A4, arrowhead) desmin, as well as multiterminal en plaque motor endings (B1–B5, arrowheads) with unchanged or slightly more intense desmin immunolabeling (B4, B5, arrowheads). Slow myofibers displayed single en plaque (C1–D5, arrowheads) as well as en grappe NMJs (E1–F5), with desmin unchanged near single en plaque motor endings (C4, C5, D4, D5) and occasionally lacking desmin in the vicinity of en grappe motor endings (E4, E5, open arrowhead). Slow myofibers lacking desmin displaying en grappe NMJs were also identified (F1–F5, arrowheads). Scale bars: 10 µm.