Table 2.
Changes in the Vitreous Humor Proteome of Diabetic Mice with and without sgp130Fc Treatment as Compared to Healthy Controls
| Accession | Symbol | Description | Fold Change Diabetic * | FDR | Fold Change Diab + Sgp130Fc † | FDR |
|---|---|---|---|---|---|---|
| P99024 | Tubb5 | Tubulin beta-5 chain | 42.45 | 4.13E-07 | 28.89 | 1.18E-04 |
| P62259 | Ywhae | 14-3-3 protein epsilon | 37.66 | 8.25E-07 | 28.65 | 1.18E-04 |
| P68372 | Tubb4b | Tubulin beta-4B chain | 37.58 | 8.25E-07 | 9.44 | 0.2751 |
| P17183 | Eno2 | Gamma-enolase | 37.58 | 8.25E-07 | 8.36 | 0.4094 |
| Q7TMM9 | Tubb2a | Tubulin beta-2A chain | 36.66 | 1.32E-06 | 21.16 | 0.0026 |
| P68254 | Ywhaq | 14-3-3 protein theta | 35.03 | 2.20E-06 | 26.02 | 3.08E-04 |
| Q8CIX8 | Lgsn | Lengsin | 32.02 | 6.79E-06 | 30.28 | 1.18E-04 |
| P15105 | Glul | Glutamine synthetase | 31.83 | 1.06E-05 | 12.53 | 0.1065 |
| P05213 | Tuba1b | Tubulin alpha-1B chain | 29.11 | 3.63E-05 | 1.00 | 1.0000 |
| P15409 | Rho | Rhodopsin | 28.36 | 3.63E-05 | 11.33 | 0.1065 |
| P24549 | Aldh1a1 | Retinal dehydrogenase 1 | 27.65 | 6.68E-05 | 21.01 | 0.0026 |
| Q9CQV8 | Ywhab | 14-3-3 protein beta/alpha | 26.68 | 9.39E-05 | 12.34 | 0.0767 |
| P61982 | Ywhag | 14-3-3 protein gamma | 26.59 | 9.74E-05 | 19.27 | 0.0087 |
| P68369 | Tuba1a | Tubulin alpha-1A chain | 25.02 | 1.75E-04 | 23.20 | 0.0016 |
| Q00623 | Apoa1 | Apolipoprotein A-I | 24.21 | 3.03E-04 | 3.68 | 1.0000 |
| Q01853 | Vcp | Transitional endoplasmic reticulum ATPase | 23.82 | 3.03E-04 | 19.20 | 0.0087 |
| Q6NVD9 | Bfsp2 | Phakinin | 23.76 | 3.03E-04 | 23.51 | 9.08E-04 |
| P26040 | Ezr | Ezrin | 21.04 | 8.72E-04 | 10.48 | 0.1628 |
| P21614 | Gc | Vitamin D binding protein | 20.93 | 8.94E-04 | 6.10 | 0.5569 |
| P50396 | Gdi1 | Rab GDP dissociation inhibitor alpha | 20.76 | 8.72E-04 | 10.25 | 0.1628 |
| O08553 | Dpysl2 | Dihydropyrimidinase-related protein 2 | 20.06 | 8.72E-04 | 5.45 | 0.7703 |
| P16125 | Ldhb | L-lactate dehydrogenase B chain | 19.13 | 0.0016 | 12.02 | 0.1065 |
| P22599 | Serpina1b | Alpha-1-antitrypsin 1-2 | 18.59 | 0.0027 | 1.00 | 1.0000 |
| P16546 | Sptan1 | Spectrin alpha chain, nonerythrocytic 1 | 18.20 | 0.0027 | 11.67 | 0.1065 |
| P16460 | Ass1 | Argininosuccinate synthase | 17.52 | 0.0027 | 15.24 | 0.0443 |
| Q78ZA7 | Nap1l4 | Nucleosome assembly protein 1–like 4 | 17.43 | 0.0027 | 13.73 | 0.0767 |
| O35945 | Aldh1a7 | Aldehyde dehydrogenase, cytosolic 1 | 16.92 | 0.0050 | 1.00 | 1.0000 |
| P01027 | C3 | Complement C3 | 16.38 | 0.0082 | 1.00 | 1.0000 |
| P48774 | Gstm5 | Glutathione S-transferase Mu 5 | 16.33 | 0.0049 | 3.68 | 1.0000 |
| Q61838 | A2m | Alpha-2-macroglobulin | 16.28 | 0.0082 | 2.34 | 1.0000 |
| P13020 | Gsn | Gelsolin | 15.55 | 0.0082 | 14.01 | 0.0443 |
| Q61753 | Phgdh | D-3-phosphoglycerate dehydrogenase | 15.22 | 0.0082 | 11.04 | 0.1628 |
| P58252 | Eef2 | Elongation factor 2 | 14.51 | 0.0153 | 7.14 | 0.4094 |
| Q62433 | Ndrg1 | Protein NDRG1 | 14.05 | 0.0153 | 9.27 | 0.2751 |
| P15499 | Prph2 | Peripherin-2 | 13.93 | 0.0153 | 4.11 | 1.0000 |
| Q02053 | Uba1 | Ubiquitin-like modifier-activating enzyme 1 | 13.22 | 0.0271 | 4.11 | 1.0000 |
| P06745 | Gpi | Glucose-6-phosphate isomerase | 13.18 | 8.80E-05 | 6.65 | 0.1065 |
| P29699 | Ahsg | Alpha-2-HS-glycoprotein | 12.69 | 1.27E-04 | 4.15 | 0.5569 |
| Q9WU62 | Incenp | Inner centromere protein | 12.56 | 0.0271 | 13.49 | 0.0767 |
| P70168 | Kpnb1 | Importin subunit beta-1 | 12.42 | 0.0271 | 5.80 | 0.5569 |
| Q6P1F6 | Ppp2r2a | Serine/threonine-protein phosphatase 2A 55 Bα | 11.74 | 0.0488 | 9.27 | 0.2751 |
| Q80ZQ5 | Jazf1 | Juxtaposed with another zinc finger protein 1 | 11.72 | 0.0488 | 7.70 | 0.4094 |
| P62874 | Gnb1 | Guanine nucleotide-binding protein subunit β-1 | 11.40 | 3.72E-04 | 4.82 | 0.3183 |
| Q9ES00 | Ube4b | Ubiquitin conjugation factor E4 B | 11.18 | 0.0488 | 9.34 | 0.2751 |
| P14602 | Hspb1 | Heat shock protein beta-1 | 9.23 | 9.15E-05 | 13.12 | 4.54E-06 |
| A2AMT1 | Bfsp1 | Filensin | 8.75 | 9.71E-04 | 7.97 | 0.0136 |
| Q91XV3 | Basp1 | Brain acid soluble protein 1 | 8.67 | 0.0050 | 6.05 | 0.1471 |
| P52480 | Pkm | Pyruvate kinase PKM | 8.29 | 3.58E-06 | 7.23 | 1.72E-04 |
| P05063 | Aldoc | Fructose-bisphosphate aldolase C | 8.20 | 3.09E-05 | 7.09 | 9.08E-04 |
| P20152 | Vim | Vimentin | 7.94 | 5.68E-04 | 5.28 | 0.0784 |
| P63017 | Hspa8 | Heat shock cognate 71-kDa protein | 7.05 | 5.68E-04 | 3.41 | 0.3183 |
| Q61171 | Prdx2 | Peroxiredoxin-2 | 6.20 | 0.0026 | 3.40 | 0.4131 |
| P49194 | Rbp3 | Retinol-binding protein 3 | 6.10 | 5.59E-06 | 3.71 | 0.0405 |
| P11499 | Hsp90ab1 | Heat shock protein HSP 90-beta | 6.03 | 0.0066 | 3.72 | 0.4131 |
| P34884 | Mif | Macrophage migration inhibitory factor | 5.96 | 0.0106 | 2.98 | 0.7414 |
| P63268 | Actg2 | Actin, gamma-enteric smooth muscle | 5.13 | 5.68E-04 | 4.11 | 0.0406 |
| Q9D1U0 | Grifin | Grifin | 4.65 | 0.0081 | 3.18 | 0.2751 |
| P20443 | Sag | S-arrestin | 4.55 | 9.15E-05 | 2.69 | 0.1709 |
| P20612 | Gnat1 | Guanine nucleotide-binding protein subunit α-1 | 4.54 | 0.0291 | –1.31 | 1.0000 |
| P07901 | Hsp90aa1 | Heat shock protein HSP 90-alpha | 4.50 | 0.0488 | 2.19 | 1.0000 |
| P05064 | Aldoa | Fructose-bisphosphate aldolase A | 4.38 | 0.0024 | 2.68 | 0.3359 |
| P10126 | Eef1a1 | Elongation factor 1–alpha 1 | 4.25 | 0.0033 | 3.14 | 0.1726 |
| Q9JI02 | Scgb2b20 | Secretoglobin family 2B member 20 | 4.02 | 0.0051 | –1.39 | 1.0000 |
| Q03734 | Serpina3m | Serine protease inhibitor A3M | 3.47 | 0.0184 | –1.23 | 1.0000 |
| P40142 | Tkt | Transketolase | 3.34 | 0.0020 | 2.64 | 0.1065 |
| Q8BFZ3 | Actbl2 | Beta-actin-like protein 2 | 3.11 | 0.0056 | 1.14 | 1.0000 |
| P06151 | Ldha | L-lactate dehydrogenase A chain | 2.87 | 0.0167 | 1.81 | 0.7448 |
| P63101 | Ywhaz | 14-3-3 protein zeta/delta | 2.43 | 0.0352 | 2.46 | 0.1065 |
| P16858 | Gapdh | Glyceraldehyde-3-phosphate dehydrogenase | 2.33 | 0.0233 | 2.13 | 0.1389 |
| P23927 | Cryab | Alpha-crystallin B chain | 2.21 | 0.0294 | 2.42 | 0.0360 |
| P11590 | Mup4 | Major urinary protein 4 | –2.83 | 0.0241 | –3.18 | 0.0352 |
| P04938 | Mup8 | Major urinary proteins 11 and 8 (fragment) | –9.94 | 0.0029 | –4.01 | 0.0784 |
FDR, false discovery rate.
*
Diabetic versus controls.
†
Diabetic with sgp130Fc treatment versus controls.