Figure 6.

MPE reduces glucotoxicity and improves β-cell function by reducing inflammation in MIN6 cells. MPE attenuates IL-1β–induced inflammation in high-glucose medium, which is inhibited by IKK inhibitor (PS1145) in MIN6 cells. A, GSIS was performed to evaluate the anti-inflammatory effect of MPE in MIN6 cells with low-glucose or high-glucose incubation. In high-glucose incubation, MIN6 cells were divided into eight groups for treatment with control or MPE, control + PS1145 or MPE + PS1145, control + IL-1β or MPE + IL-1β, and control + IL-1β + PS1145 or MPE + IL-1β + PS1145. B, ATP/ADP ratio was measured in different cell groups using the same treatments as in A. C, relative RNA expression of il1b was measured in control or MPE, control + IL-1β or MPE + IL-1β, and control + IL-1β + PS1145 or MPE + IL-1β + PS1145. D, IL-1β protein levels from supernatant were measured in different cell groups using same treatments as in C. Data are presented as mean ± S.E. (error bars) with individual data points in histograms. *, p < 0.05.