Table 1.
SAXS parameters
| DH/PH | DH/PH-DEP1 | p-DH/PH-DEP1 | |
|---|---|---|---|
| Guinier analysis | |||
| I(0)a | 0.003 ± 4.8E-06 | 0.0027 ± 0.00013 | 0.0027 ± 3.4E-06 |
| Rg (nm) | 2.84 ± 0.009 | 2.99 ± 0.009 | 2.96 ± 0.007 |
| Qmin (nm−1) | 0.043 | 0.043 | 0.043 |
| Qmax (nm−1) | 0.430 | 0.422 | 0.433 |
| P(r) analysis | |||
| Dmax (nm) | 10.5 | 10.4 | 10.5 |
| Volume (nm3) | 95.2 | 118 | 117 |
| MMexp (MMcal) (kDa) | 47 (43) | 59 (53.7) | 59 (53.7) |
| EOM analysis | |||
| Crystal structure | 5FI1 | 5FI1 and 6VSK | 5FI1 and 6VSK |
| q-Range for fitting (nm−1) | 0.043-3.55 | 0.043-3.55 | 0.043-3.55 |
| Symmetry assumptions | None | None | None |
| χ2 | 1.0 | 1.1 | 1.0 |
| Constant subtraction | 0.0 | 0.0 | 0.0 |
| No. of representative models | 3 | 5 | 4 |
| Ensemble (pool) average Rg (nm) | 2.8 (2.8) | 3.0 (3.1) | 3.0 (3.1) |
| Ensemble (pool) average Dmax (nm) | 9.9 (9.0) | 10. (10.) | 10. (10.) |
| Ensemble (pool) average volume (nm3) | 83 (79) | 104 (102) | 103 (102) |
| Ensemble (pool) average Cα(N)-Cα(C) distance (nm) | 7.2 (6.0) | 6.3 (6.8) | 6.4 (6.8) |
| Rflex ensemble (pool) | 86% (87%) | 77% (84%) | 73% (84%) |
| Rσ | 6.0 | 4.5 | 3.4 |
a I(0), Rg, Dmax, Qmin, Qmax, MMexp, MMcal, χ2, Rflex, and Rσ are the experimentally determined intensity at zero scattering angle, radius of gyration, maximum particle dimension in the Guinier fit, minimum scattering angle in the Guinier fit, maximum scattering angle, molecular mass calculated from scattering data, molecular mass based on amino acid sequence, χ squared fit between the theoretical scattering of selected ensemble and the experimental SAXS data, flexibility metric of ensemble compared with pool (value in parentheses), ratio of standard deviation for the distribution of selected ensemble to that of the pool, respectively.