Figure 5.

The N-glycan signatures differ on soluble (luminal) and membrane proteins in the different neutrophil organelles. Quantitative glycomics was performed for N-glycans released from separated membrane and lumen (soluble) protein extracts from isolated neutrophil granules and SV + PM. The identified glycans have been grouped and quantified according to their N-glycan type, i.e. paucimannosidic, oligomannosidic, and complex N-glycans. Note that the glycans from the soluble proteins in the SV + PM fraction are not of neutrophil origin but come from plasma proteins. The data represent the means ± S.E., and unpaired two-way Student's t test was used for statistical analysis. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.