Dominant core 2 O-glycosylation in SG and GG fractions.
a, core 2 O-glycans were commonly observed with LacNAc extensions on the 6′ arm and sialic acid capping on the 3′ arm galactose in SG + GG fractions. b, LC–MS/MS spectra of core 2 O-glycan (m/z 11861−) with sialic acid capping on the 3′ arm and Lewis epitope on the 6′ arm. Asterisks represent contaminating signals of unknown origin. Blue squares, GlcNAc; yellow squares, GalNAc; red triangles, fucose; yellow circle, galactose; purple diamonds, sialic acid (N-acetylneuraminic acid).