Figure 7.

Overview of glycosylation-by-timing hypothesis. Granule formation during neutrophil maturation is a continuum, and granules carry a vast array of glycans. From the presence of shorter paucimannosidic glycans in AG, to ever-elongating complex glycans in SG, and subsequently in GG and the corresponding mRNA expression of different glycosidases and glycosyltransferases, suggests an evolution of granule glycosylation over time. Similar to the targeting-by-timing hypothesis describing the temporal protein synthesis of maturing neutrophils, we hypothesize a parallel glycosylation-by-timing granule glycan synthesis that takes place during neutrophil maturation.