Figure 1.

Solution characterization of the PE5–PPE4–EspG₃ heterotrimer. A, schematic showing design and molecular masses for constructs used in this study. PE5 from M. tuberculosis (Rv0285) contains an N-terminal His₆ tag that is connected to the gene via a TEV protease cleavable linker. PPE4 (Rv0286) was truncated after its N-terminal PPE domain. Full-length copies of both M. tuberculosis (Rv0289) and M. marinum (MMAR_0548) EspG₃ were used. B and C, elution profile of PE5_mt–PPE4_mt–EspG_3mt (B) and PE5_mt–PPE4_mt–EspG_3mm (C), with the right y axis showing the MALS-measured molecular mass. The insets show an SDS-PAGE image of the major peak fraction. res., residues.