Skip to main content
. 2020 Aug 14;9:e52558. doi: 10.7554/eLife.52558

Figure 1. Transcriptome and metabolomics analysis of brown fat shows increased carbohydrate metabolism and glycolytic metabolism during cold exposure.

(a) Network visualization of enriched biological pathways altered with cold exposure in BAT (N = 5). (b) GSEA pathway analysis of differentially expressing genes (FDR < 0.05) in BAT at 4°C versus room temperature (N = 5). (c) Heat map of relative normalized changes in BAT (c) and serum (e) metabolites at 30°C, 23°C, and 4°C. Dendograms illustrate hierarchical clustering of pattern similarity across metabolites (left) and conditions (top). Each column represents average within the group (N = 5 per group). Data was sum normalized, log transformed, and autoscaled. (d) MSEA pathway analysis of metabolites in BAT (d) and serum (f) from mice at 4°C versus room temperature (N = 5).

Figure 1.

Figure 1—figure supplement 1. RNRNA-seq analysis of brown adipose tissue from mice at room temperature or with cold exposure.

Figure 1—figure supplement 1.

(a) Hierarchical clustering illustrates large-scale differences in gene expression between cold room and room temperature exposed BAT. (b) Unsupervised principal component analysis (PCA) shows that cold room and room temperature exposed BATs are separated and form distinctive clusters in principal components. The first three principal components were used to present the samples in the 3-dimentional PCA plot.