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. 2020 Aug 14;9:e52558. doi: 10.7554/eLife.52558

Figure 3. Loss of MPC1 in BAT impairs thermogenesis and leads to cold sensitivity.

(a) Relative gene expression in brown adipose tissue from mice adapted to 30°C or 4°C for 1 day or 1 week. N = 4–5. (b) Relative gene expression in brown adipose tissue from mice adapted to 30°C or 23°C for 1 week. N = 4–5 (c) Western blot analysis of brown adipose tissue of mice adapted to 30°C or 4°C for 1 week. N = 4–5. (d) Gene expression of MPC1 and MPC2 in brown adipose tissue after 6 hr of cold exposure. N = 7. (e) Western blot analysis of brown adipose tissue and white adipose tissue (f) at 4°C. N = 4. (g) Core body temperature during cold challenge at 4°C. N = 7.

Figure 3.

Figure 3—figure supplement 1. Regulation of Mpc1 in response to the cold and the conditional deletion of Mpc1 in brown fat.

Figure 3—figure supplement 1.

(a) Quantification of western blot analysis of brown adipose tissue of mice adapted to 30°C or 4°C for 1 week (N = 4–5), (p<0.05). (b) Quantification of western blot analysis of brown adipose tissue (b) and white adipose tissue (c) at 4°C from Mpc1F/F and Mpc1F/F::Ucp1Cre mice (N = 4). (d) Blood glucose levels in Mpc1F/F and Mpc1F/F::Ucp1Cre mice during cold challenge at 4°C. N = 6.