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. 2020 Aug 14;9:e52558. doi: 10.7554/eLife.52558

Figure 4. Conditional deletion of Mpc1 in BAT impairs systemic glucose metabolism.

(a) Glucose tolerance test at room temperature (23°C) and cold (4°C) in Mpc1F/F and Mpc1F/F::Ucp1Cre3–4 months old, N = 5. (b) Insulin tolerance test at room temperature (23°C) in Mpc1F/F and Mpc1F/F::Ucp1Cre, 3–4 months old, N = 6. (c). Representative H and E images of BAT, iWAT, eWAT, and liver from Mpc1F/F and Mpc1F/F::Ucp1Cre mice exposed to 4°C for 6 hr. (d) Gene expression in BAT from Mpc1F/F and Mpc1F/F::Ucp1Cre mice exposed to 4°C for 6 hr. N = 6. (e–g) Energy expenditure, RER, and locomotor activity of Mpc1F/F and Mpc1F/F::Ucp1Cre mice at 6°C. N = 4.

Figure 4.

Figure 4—figure supplement 1. Conditional deletion of Mpc1 in BAT and the impact on body composition and blood glucose.

Figure 4—figure supplement 1.

(a) Body composition of Mpc1F/F and Mpc1F/F::Ucp1Cre mice housed at room temperature by NMR. (b) Time-dependent changes in blood glucose levels in Mpc1F/F and Mpc1F/F::Ucp1Cre mice mice 3–4 months old treated with 1 mg/kg CL-316,243 (N = 6). (c) Gene expression in BAT from Mpc1F/F and Mpc1F/F::Ucp1Cre mice housed at 4°C for 6 hr. (d) Gene expression in iWAT from Mpc1F/F and Mpc1F/F::Ucp1Cre mice housed at 4°C for 6 hr.