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. 2020 Aug 14;9:e52558. doi: 10.7554/eLife.52558

Figure 8. Conditional deletion of Mpc1 in brown fat leads to increased ketogenesis.

(a) Volcano plot showing changes in serum metabolites between Mpc1F/F and Mpc1F/F::Ucp1Cre mice housed at 4°C for 6 hr. N = 6. (b) Heat map of top 25 metabolites in BAT from Mpc1F/F and Mpc1F/F::Ucp1Cre mice housed at 4°C for 6 hr. Dendograms illustrate hierarchical clustering across metabolites (left) and genotypes (top). N = 6. Data was sum normalized, log transformed, and autoscaled. (c) Serum 3-hydroxybutyrate levels from Mpc1F/F and Mpc1F/F::Ucp1Cre mice housed at 23°C or 4°C for 6 hr. N = 6. (d) Gene expression analysis of livers from Mpc1F/F and Mpc1F/F::Ucp1Cre mice housed at 4°C for 6 hr. N = 6.

Figure 8.

Figure 8—figure supplement 1. Gene expression analysis and serum FFA levels.

Figure 8—figure supplement 1.

(a) Gene expression in liver from Mpc1F/F and Mpc1F/F::Ucp1Cre mice housed at 4°C for 6 hr. Data was analyzed by Student’s t-test. Values are shown as mean + s.e.m. (p<0.05). (b) Free fatty acid levels in serum of Mpc1F/F and Mpc1F/F::Ucp1Cre mice housed at 4°C for 6 hr (N = 6) (c) Ketogenic gene expression in iWAT from Mpc1F/F and Mpc1F/F::Ucp1Cre mice housed at 4°C for 6 hr. Data was analyzed by Student’s t-test. Values are shown as mean + s.e.m. (N = 6).