Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Biological sample (Human) | primary human umbilical vein endothelial cells | Leiden University Medical Center (LUMC) | freshly isolated from umbilical cord of male newborns | |
| Chemical compound, drug | phorbol 12-myristate 13-acetate | Sigma-Aldrich | Cat#:P8139 | |
| Peptide, recombinant protein |
tumor necrosis factor-α | Sigma-Aldrich | Cat#:H8916 | |
| Biological sample (Rat) | rat tail collagen type 1 | Trevigen | Cat#:3440-005-01 | |
| Antibody | mouse anti-human CD144 | BD Biosciences | Cat#:555661; RRID:AB_396015 | IF(1:150) |
| Antibody | sheep anti-human CD31 | R and D Systems | Cat#:AF806; RRID:AB_355617 | IF(1:150) |
| Antibody | rabbit anti-human vWF | Agilent Dako | Cat#:A0082; RRID:AB_2315602 | IF(1:1000) |
| Antibody | alexa fluor 488-conjugated goat anti-mouse | ThermoFisher | Cat#:R37120; RRID:AB_2556548 | IF(1:250) |
| Antibody | alexa fluor 488-conjugated donkey anti-sheep | ThermoFisher | Cat#:A11015; RRID:AB_141362 | IF(1:250) |
| Antibody | alexa fluor 647-conjugated goat anti-rabbit | ThermoFisher | Cat#:A27040; RRID:AB_2536101 | IF(1:250) |
| Other | rhodamine phalloidin | Sigma-Aldrich | Cat#:P1951; RRID:AB_2315148 | IF(1:200) |
| Other | hoechst | Invitrogen | Cat#:H3569; RRID:AB_2651133 | IF(1:2000) |
| Software, algorithm | LabSolutions | Shimadzu | RRID:SCR_018241 | |
| Software, algorithm | SPSS | SPSS | RRID:SCR_002865 | |
| Software, algorithm | GraphPad Prism | GraphPad | RRID:SCR_002798 |