Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Escherichia coli) | E. coli strains | This study | Table 2 | |
| Sequence-based reagent | This study | PCR primers | Supplementary file 2 | |
| Recombinant DNA reagent | pZASS-tdcE (plasmid) | This study | Overexpression vector of TdcE. | |
| Recombinant DNA reagent | pCA24N-metB (plasmid) | This study | Overexpression vector of MetB. | |
| Recombinant DNA reagent | pCA24N-metC (plasmid) | This study | Overexpression vector of MetC. | |
| Recombinant DNA reagent | pCA24N-cysE (plasmid) | This study | Overexpression vector of CysE. | |
| Recombinant DNA reagent | pCA24N-cysE_A33T (plasmid) | This study | Overexpression vector of CysE with a mutation A33T. | |
| Recombinant DNA reagent | pORTMAGE | Nyerges et al., 2016 | Addgene catalog no. 72680 | |
| Chemical compound, drug | 13C-1-glucose | Sigma Aldrich | ||
| Chemical compound, drug | 13C-3-glucose | Sigma Aldrich | ||
| Chemical compound, drug | Sodium propionate-1-13C | Sigma Aldrich | ||
| Commercial assay or kit | RNeasy Mini Kit | Qiagen | ||
| Commercial assay or kit | qScript cDNA Synthesis Kit | QuantaBio | ||
| Commercial assay or kit | Maxima SYBR Green/ROX qPCR Master Mix | Thermo Fisher Scientific | ||
| Software, algorithm | breseq | Deatherage and Barrick, 2014 | RRID:SCR_010810 |