Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Mus musculus) | Mouse: Nts-cre | Jackson Laboratories | Stock#017525 | N/A |
| Genetic reagent (Mus musculus) | Mouse: Cartpt-cre | Jackson Laboratories | Stock#028533 | N/A |
| Genetic reagent (Mus musculus) | Mouse: Sst-cre | Jackson Laboratories | Stock#013044 | N/A |
| Genetic reagent (Mus musculus) | Mouse: Glp1r-cre | Jackson Laboratories | Stock#029283 | N/A |
| Antibody | Anti-phospho ribosomal protein 6 pSer244/pSer247 (Rabbit polyclonal) | Invitrogen | Cat#44–923G, RRID:AB_2533798 | (1:1000) |
| Antibody | Anti-GFP (Chicken polyclonal) | Abcam | Cat#ab13970, RRID:AB_300798 | (1:1000) |
| Antibody | 19C8 and 19F7, GFP monoclonal antibodies | Memorial Sloan-Kettering Monoclonal Antibody Facility | Custom order | (50 ug) |
| Antibody | Anti-mCherry (Chicken polyclonal) | Abcam | Cat# ab205402 | (1:1000) |
| Antibody | Mouse anti-cfos (Rabbit monoclonal) | Cell Signaling | Cat#2250S, RRID:AB_2247211 | (1:500) |
| Antibody | Anti-rabbit IgG Alexa 488 (Goat polyclonal) | Invitrogen | Cat#A11008, RRID:AB_143165 | (1:1000) |
| Antibody | Anti-rabbit IgG Alexa 594 (Goat polyclonal) | Invitrogen | Cat#A11072, RRID:AB_142057 | (1:1000) |
| Antibody | Anti-chicken IgG Alexa 488 (Goat polyclonal) | Invitrogen | Cat#A11039, RRID:AB_142924 | (1:1000) |
| Antibody | Anti-chicken IgG Alexa 594 (Goat polyclonal) | Invitrogen | Cat#A11042, RRID:AB_142083 | (1:1000) |
| Recombinant DNA reagent | AAV5-EF1a-DIO-YFP | UNC Vector Core | N/A | N/A |
| recombinant DNA reagent | AAV5-EF1a-DIO-hChR2(H134R)-YFP | UNC Vector Core | N/A | N/A |
| Recombinant DNA reagent | AAV5-EF1a-DIO-hM3D(Gq)-mCherry | UNC Vector Core | N/A | N/A |
| Recombinant DNA reagent | AAV5-EF1a-DIO-mCherry | UNC Vector Core | N/A | N/A |
| Recombinant DNA reagent | AAV5-EF1a- DIO-hM4D(Gi)-mCherry | UNC Vector Core | N/A | N/A |
| Recombinant DNA reagent | AAV5-DIO-GCaMP6s | Addgene | Cat#510882 | N/A |
| Recombinant DNA reagent | HSV-lsl-tdTomato | Lo and Anderson, 2011 | N/A | N/A |
| Recombinant DNA reagent | AAV5-Introvert-GFPL10a | Nectow et al., 2017 | N/A | N/A |
| Sequence-based reagent | PrimeTime Standard qPCR Assay for NTS | idtDNA | Mm.PT.58.10351472 | N/A |
| Sequence-based reagent | PrimeTime Standard qPCR Assay for ActB | idtDNA | Mm.PT.47.5885043.g | N/A |
| Sequence-based reagent | Probe for NTS (C1) | ACDBio | Cat# 420448 | N/A |
| Sequence-based reagent | Probe for cFos (C2) | ACDBio | Cat#506921-C2 | N/A |
| Sequence-based reagent | Probe for Glp1r (C3) | ACDBio | Cat#418851-C3 | N/A |
| Sequence-based reagent | Probe for Sst (C3) | ACDBio | Cat#404631-C3 | N/A |
| Sequence-based reagent | Probe for Mc3r (C3) | ACDBio | Cat#412541-C3 | N/A |
| Sequence-based reagent | Probe for Cartpt (C3) | ACDBio | Cat#432001-C3 | N/A |
| Sequence-based reagent | Probe for vGAT (C2) | ACDBio | Cat#319191-C2 | N/A |
| Sequence-based reagent | Probe for vGLUT2 (C3) | ACDBio | Cat#319171-C3 | N/A |
| Peptide, recombinant protein | NBL10 recombinant peptide | Chromotek | Cat# GT-250 | 100 ng/mL |
| Commercial assay or kit | RNAeasy Mini kit | Qiagen | Cat#74104 | N/A |
| Commercial assay or kit | RNAscope Fluorescent Multiplex Reagent Kit V2 | ACDbio | Cat#323100 | N/A |
| Chemical compound, drug | Clozapine-N-oxide(CNO) | Tocris Biosciences | Cat#4936; CAS: 34233-69-7 | 1 mg/kg |
| Software, algorithm | Tophat | Basespace | https://basespace.illumina.com/apps/ | N/A |
| Software, algorithm | Cufflinks | Basespace | https://basespace.illumina.com/apps/ | N/A |
| Software, algorithm | SYNAPSE | Tucker-Davis Technologies | https://tdt.com | N/A |
| Software, algorithm | MATLAB | Mathworks | https://mathworks.com | N/A |
| Software, algorithm | ImageJ | NIH | https://imagej.nih.gov/ij/ | N/A |
| Software, algorithm | GraphPad Prism 5.0 | GraphPad | https://www.graphpad.com/scientific-software/prism/ | N/A |
| Software, algorithm | Ethovision 9.0 | Noldus | https://noldus.com | N/A |