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. 2020 Sep 7;9:e55732. doi: 10.7554/eLife.55732

Key resources table.

Reagent type
(species) or resource
Designation Source or reference Identifiers Additional
information
Strain, strain background Rag1 mice JAXmice 002216 - B6.129S7-Rag1 < tm1Mom>/J
Strain, strain background NODSCID mice JAXmice 001303 - NOD.Cg-Prkdc<scid > /J
Cell line Multipotent human CNS-derived stem cells Uchida et al., 2000 Stem cells inc 2491.2 Human Neural stem cells
Cell line Human Neural stem cells This paper and Piltti et al., in preparation UCI161.1 Human Neural stem cells (UCI) correspondence to aja@uci.edu
Transfected construct Chimeric gRNA +hspCAS9 co-expression Vector https://www.appliedstemcell.com/ Custom made Guide RNA sequences in Figure 5—figure supplement 1
Antibody Anti-STEM121
(mouse monoclonal)
TAKARA Cat# Y40410 RRID:AB_2801314 IF(1:3000)
Antibody Anti-STEM123
(mouse monoclonal)
TAKARA Cat# Y40420
Antibody ID:AB_2833249
IF(1:3000)
Antibody Fibronectin
(rabbit)
Sigma Aldrich Cat# F3648 RRID:AB_476976 IF(1:500)
Antibody Anti-C1q
(mouse monoclonal)
Abcam Cat#: ab71940
RRID:AB_10711046
WB (1:200)
Far-WB (1:50)
PLA (1:100)
Antibody Anti-pERK
(rabbit)
Cell Signaling Cat#: 4370 RRID:AB_10693765 WB (1:1000)
Antibody Anti-pAkt
(rabbit)
Cell Signaling Cat#: 9916
RRID:AB_10693765
WB (1:1000)
Antibody Anti-CD44
(rabbit monoclonal)
Abcam Cat#: ab51037
RRID:AB_868936
WB (1:1000)
ICC (1:500)
PLA (1:500)
Antibody Anti-CD44
(rabbit)
Abcam Cat#: ab189524
ICC (1:500)
Antibody Anti-c-MET
(rabbit monoclonal)
Abcam Cat#: ab51067
RRID:AB_880695
PLA (1:500)
Antibody Anti-BAI-1
(rabbit polyclonal)
Abcam Cat#: ab135907
PLA (1:500)
Antibody Anti-GPR62
(rabbit polyclonal)
Thermo
Fisher
Cat#: PA5-33745 RRID:AB_2551116 PLA (1:500)
Antibody Anti-ADCY5
(rabbit polyclonal)
Abcam Cat#: ab66037
RRID:AB_1140781
PLA (1:500)
Antibody Anti-Olig2
(rabbit polyclonal)
Abcam Cat#: ab136253
RRID:AB_2810961
ICC (1:1000)
Antibody Tubulin βIII
(mouse monoclonal)
Biolegend Cat#: MMS-435P RRID:AB_2313773 ICC (1:500)
Antibody GFAP
(rabbit polyclonal)
Dako Cat#: Z 0334 RRID:AB_10013382 ICC (1:1000)
Antibody Anti CD44 -PE Miltenyi Cat#:130-110-293
RRID:AB_2658165
FACS (1:11)
Antibody Anti CD133-FITC Miltenyi Cat#:130-113-673
RRID:AB_2726215
FACS (1:11)
Antibody Anti-C1qNAb (Goat-anti human) Quidel Cat#:A031 C1q neutralization in vivo [100 µg/mL] 2 µL injection
Sequence-based reagent Primers different mRNAs This paper NA Figure 5—figure supplement 1
Peptide, recombinant protein Purified human C1q My
Biosource
Cat. #: MBS143105 [0.1nM = 28 ng/mL]
[1nM = 400 ng/mL]
[200nM = 80µg/mL]
Chemical compound, drug PD98059 (pERK MAPK inhibitor) GIBCO Life Technologies Cat. #: PHZ1164 10 µM dissolved in DMSO
Chemical compound, drug SB203580 (p38 MAPK inhibitor) GIBCO Life Technologies Cat. #: PHZ1253 10 µM dissolved in DMSO
Chemical compound, drug Pertussis Toxin (GPCR inhibitor) GIBCO Life Technologies Cat. #: PHZ1174 10 µg/mL
Chemical compound, drug DMSO Sigma-Aldrich Cat. #: D26650 1:1000
Chemical compound, drug PathScan Intracellular signaling array kit Cell Signaling Cat. #7744 Phosphoarray
Cell signalling
Chemical compound, drug Pierce Pull-Down biotinylated protein-protein interaction kit; Thermo Cat. #21115 Protein pulldown
Chemical compound, drug RNeasy Mini Kit Qiagen Cat No./ID: 74104 RNA extraction
Chemical compound, drug DNA-free DNase I (Ambion) Thermo Fisher Cat. # AM2224 DNA elimination
Chemical compound, drug High Capacity cDNA Reverse Transcription Kit ABI Life Technologies Cat. # 4368814 Retrotranscription
Chemical compound, drug Duolink in situ Red starter kit Mouse/Rabbit Sigma Cat. # DUO92101 Proximity Ligation Assay (PLA)
Chemical compound, drug QCM Chemotaxis Cell Migration Assay, 96-well Millipore/
Chemicon
Cat. # ECM510 Cell migration
Software, algorithm SnapGene SnapGene RRID:SCR_015052 Sequencing
Software, algorithm StereoInvestigator Microbrightfield Stereology analysis
Software, algorithm ImageJ ImageJ RRID:SCR_003070 WB data analysis
Software, algorithm Prism8 Graphpad RRID:SCR_002798 Statistics and graphs
Other Hoechst 33342 Invitrogen
Thermo
H3569 1:1000