Key resources table.
Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Strain, strain background | Rag1 mice | JAXmice | 002216 - B6.129S7-Rag1 < tm1Mom>/J | |
Strain, strain background | NODSCID mice | JAXmice | 001303 - NOD.Cg-Prkdc<scid > /J | |
Cell line | Multipotent human CNS-derived stem cells | Uchida et al., 2000 | Stem cells inc 2491.2 | Human Neural stem cells |
Cell line | Human Neural stem cells | This paper and Piltti et al., in preparation | UCI161.1 | Human Neural stem cells (UCI) correspondence to aja@uci.edu |
Transfected construct | Chimeric gRNA +hspCAS9 co-expression Vector | https://www.appliedstemcell.com/ | Custom made | Guide RNA sequences in Figure 5—figure supplement 1 |
Antibody | Anti-STEM121 (mouse monoclonal) |
TAKARA | Cat# Y40410 RRID:AB_2801314 | IF(1:3000) |
Antibody | Anti-STEM123 (mouse monoclonal) |
TAKARA | Cat# Y40420 Antibody ID:AB_2833249 |
IF(1:3000) |
Antibody | Fibronectin (rabbit) |
Sigma Aldrich | Cat# F3648 RRID:AB_476976 | IF(1:500) |
Antibody | Anti-C1q (mouse monoclonal) |
Abcam | Cat#: ab71940 RRID:AB_10711046 |
WB (1:200) Far-WB (1:50) PLA (1:100) |
Antibody | Anti-pERK (rabbit) |
Cell Signaling | Cat#: 4370 RRID:AB_10693765 | WB (1:1000) |
Antibody | Anti-pAkt (rabbit) |
Cell Signaling | Cat#: 9916 RRID:AB_10693765 |
WB (1:1000) |
Antibody | Anti-CD44 (rabbit monoclonal) |
Abcam | Cat#: ab51037 RRID:AB_868936 |
WB (1:1000) ICC (1:500) PLA (1:500) |
Antibody | Anti-CD44 (rabbit) |
Abcam | Cat#: ab189524 |
ICC (1:500) |
Antibody | Anti-c-MET (rabbit monoclonal) |
Abcam | Cat#: ab51067 RRID:AB_880695 |
PLA (1:500) |
Antibody | Anti-BAI-1 (rabbit polyclonal) |
Abcam | Cat#: ab135907 |
PLA (1:500) |
Antibody | Anti-GPR62 (rabbit polyclonal) |
Thermo Fisher |
Cat#: PA5-33745 RRID:AB_2551116 | PLA (1:500) |
Antibody | Anti-ADCY5 (rabbit polyclonal) |
Abcam | Cat#: ab66037 RRID:AB_1140781 |
PLA (1:500) |
Antibody | Anti-Olig2 (rabbit polyclonal) |
Abcam | Cat#: ab136253 RRID:AB_2810961 |
ICC (1:1000) |
Antibody | Tubulin βIII (mouse monoclonal) |
Biolegend | Cat#: MMS-435P RRID:AB_2313773 | ICC (1:500) |
Antibody | GFAP (rabbit polyclonal) |
Dako | Cat#: Z 0334 RRID:AB_10013382 | ICC (1:1000) |
Antibody | Anti CD44 -PE | Miltenyi | Cat#:130-110-293 RRID:AB_2658165 |
FACS (1:11) |
Antibody | Anti CD133-FITC | Miltenyi | Cat#:130-113-673 RRID:AB_2726215 |
FACS (1:11) |
Antibody | Anti-C1qNAb (Goat-anti human) | Quidel | Cat#:A031 | C1q neutralization in vivo [100 µg/mL] 2 µL injection |
Sequence-based reagent | Primers different mRNAs | This paper | NA | Figure 5—figure supplement 1 |
Peptide, recombinant protein | Purified human C1q | My Biosource |
Cat. #: MBS143105 | [0.1nM = 28 ng/mL] [1nM = 400 ng/mL] [200nM = 80µg/mL] |
Chemical compound, drug | PD98059 (pERK MAPK inhibitor) | GIBCO Life Technologies | Cat. #: PHZ1164 | 10 µM dissolved in DMSO |
Chemical compound, drug | SB203580 (p38 MAPK inhibitor) | GIBCO Life Technologies | Cat. #: PHZ1253 | 10 µM dissolved in DMSO |
Chemical compound, drug | Pertussis Toxin (GPCR inhibitor) | GIBCO Life Technologies | Cat. #: PHZ1174 | 10 µg/mL |
Chemical compound, drug | DMSO | Sigma-Aldrich | Cat. #: D26650 | 1:1000 |
Chemical compound, drug | PathScan Intracellular signaling array kit | Cell Signaling | Cat. #7744 | Phosphoarray Cell signalling |
Chemical compound, drug | Pierce Pull-Down biotinylated protein-protein interaction kit; | Thermo | Cat. #21115 | Protein pulldown |
Chemical compound, drug | RNeasy Mini Kit | Qiagen | Cat No./ID: 74104 | RNA extraction |
Chemical compound, drug | DNA-free DNase I (Ambion) | Thermo Fisher | Cat. # AM2224 | DNA elimination |
Chemical compound, drug | High Capacity cDNA Reverse Transcription Kit | ABI Life Technologies | Cat. # 4368814 | Retrotranscription |
Chemical compound, drug | Duolink in situ Red starter kit Mouse/Rabbit | Sigma | Cat. # DUO92101 | Proximity Ligation Assay (PLA) |
Chemical compound, drug | QCM Chemotaxis Cell Migration Assay, 96-well | Millipore/ Chemicon |
Cat. # ECM510 | Cell migration |
Software, algorithm | SnapGene | SnapGene | RRID:SCR_015052 | Sequencing |
Software, algorithm | StereoInvestigator | Microbrightfield | Stereology analysis | |
Software, algorithm | ImageJ | ImageJ | RRID:SCR_003070 | WB data analysis |
Software, algorithm | Prism8 | Graphpad | RRID:SCR_002798 | Statistics and graphs |
Other | Hoechst 33342 | Invitrogen Thermo |
H3569 | 1:1000 |