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Short print-out protocol for RNAscope and BaseScope assays on fresh-frozen and paraffin sections as well as on adherent cell culture samples. For detailed protocols please refer to the text. Drops illustrate washing steps of varying lengths with indicated solutions, and the pen indicates the drawing of the hydrophobic barrier. The BaseScope assay requires an additional H2O2 pretreatment step (highlighted in light violet in top panels). Protocols can be paused (red stop signs).
